The evaluation for CpG island methylation of FASN and SREBP promoter in Mongolian gerbils NAFLD model

Introduction The methylation of CpG island in promoter and its nearby area is one the most important way to inhibit gene expression. aim this study was examine potential relationship between for fatty acid synthase (FASN) sterol regulatory element-binding protein 1 (SREBP-1). Material methods Ten newborn pups gerbils as NB group, 10 adult with normal diet control adults high-fat NAFLD 8-month-old aged group. Blood liver samples were collected serum lipid detection histopathology. A pyrosequencing technique employed determine rate. Then, transcription expression level FASN SREBP-1 verified. Results Serum chelosterol triglyceride significantly increased group (vs. control, P<0.05). also showed obvious hepatic steatosis confirmed by histological examination. Control had highest rate SREBP-1, which reduced Except both levels genes presented group> Aged > Genes SREBP a trend hypomethylation gerbil model. Conclusions tended decrease, while increase age disease development. might be new method evaluate animal model an available target genetic marker screening.